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생약 제제가 세포활성도에 미치는 효과
Effects of some herbal drugs on gingival fibroblast and periodontal ligament celltdar activity

대한치주과학회지 1997년 27권 3호 p.459 ~ 468

두진수, 강정구, 김수남, 김수남,

소속 상세정보

두진수 (  ) - 원광대학교 치과대학 치주과학교실
강정구 (  ) - 원광대학교 치과대학 치주과학교실
김수남 (  ) - 원광대학교 치과대학 치주과학교실
김수남 (  ) - 원광대학교 치과대학 치주과학교실

KMID : 0363019970270030459

Abstract

Healing of periodontal tissues require the migration and proliferation of gingival fibroblasts and periodontal ligament cells. There is many evidences that the some agents like cytokines and polypeptide growth factors are mediate these cellular events in wound healing. Recently someone is interested in herbal drugs on periodontal tissue healing processes. The purpose of this study was to examine the effects of 4 herbal drugs, Carthami Flis, Moutan Radicis Cortex, ScM Rhisoma, Seed of Carthamus tinctaius L. on human gingival fibroblasts and periodontal ligament cells. Periodontal ligament cells and gingival fibroblasts were primarily cultured from extracted premolar with non-periodontal diseases. The powder from extracted herbal drugs were prepared with distilled water. Cells were cultured with DMEM at 37´C, 5% COz, 100% humidity incubator, and treated with each herbal drugs with proper concentration for 1, 2, and 3 days. The cell activity was determined by ELISA reader using MTT assay.
There was the most significant elevation in 10-3g/sl of almost herbal. drugs on cellular activities. The result of this study demonstrated that Carthami Flis, Moutan Radicis Cortex, Scirpi Rhisoma, Seed of Carthamus tinctorius L. appears to have beneficial effect on healing process after periodontal treatment.